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- Bacto-tryptone  20 gram (g)
- Bacto-yeast extract  5 gram (g)
- NaCl 0.5 gram (g)
- 1M KCl 2.5 millilitre (ml)
ddH2O to 1 liter.
Total volume 1 liter.
Note: adjust pH to 7.0 with 10N NaOH, autoclave to sterilize, add 20 ml of sterile 1 M glucose immediately before use.         


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Submitter Laia Bahima
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protocol details
protocol id 54
protocol views  2432
Added 2007.10.27
 

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